Is P1 phage lysogenic?
P1 is a temperate bacteriophage that infects Escherichia coli and some other bacteria. When undergoing a lysogenic cycle the phage genome exists as a plasmid in the bacterium unlike other phages (e.g. the lambda phage) that integrate into the host DNA.
Is P1 a temperate phage?
P1 is a temperate bacteriophage (phage) that infects Escherichia coli and some other bacteria. A unique feature of phage P1 is that during lysogeny its genome is not incorporated into the bacterial chromosome, as is commonly observed during lysogeny of other bacteriophage.
How does P1 transduction work?
P1 can carry two or more different genetic markers in the same transducing particle DNA, and at some frequency both of these markers can be recombined into the recipient strain. Such markers are said to be linked by P1 transduction, i.e., they are close enough to be co-transduced.
What is P1 gene?
P1 genes expressed in the lytic pathway are those involved in the timing of phage development, in replication (from a “lytic” origin different from oriR), in the formation of phage particles, including headful packaging, and finally in cell lysis to release the phage progeny.
What is PAC vector?
A P1-derived artificial chromosome, or PAC, is a DNA construct derived from the DNA of P1 bacteriophages and Bacterial artificial chromosome. It can carry large amounts (about 100–300 kilobases) of other sequences for a variety of bioengineering purposes in bacteria.
What is the lytic and lysogenic cycle?
The lytic cycle involves the reproduction of viruses using a host cell to manufacture more viruses; the viruses then burst out of the cell. The lysogenic cycle involves the incorporation of the viral genome into the host cell genome, infecting it from within.
What is a virulent phage?
virulent phage A bacteriophage that causes the destruction of the host bacterium by lysis. Temperate phages, on the other hand, rarely cause lysis. A Dictionary of Plant Sciences.
How are temperate and virulent the same?
Temperate phages replicate via both lytic and lysogenic cycles. Virulent phages show generalized transduction, and they are capable of killing the host bacterium after each infection cycle. Temperate viruses show specialized transduction, and they do not kill the host bacterium immediately after the infection.
What is the purpose of colony hybridization?
Colony hybridization is the blot analysis technique where the bacterial cells are transferred from the solid nutrient medium to the absorbent material. It can define the method for isolating the specific DNA sequences or genes from the bacterial cells containing hybrid DNA using a nitrocellulose membrane filter.
Which type of vector is P1?
Later on, P1 was developed as a cloning vector by Nat Sternberg and colleagues in the 1990s. It is capable of Cre-Lox recombination. The P1 vector system was first developed to carry relatively large DNA fragments in plasmids (95-100kb).
What is a PAC site?
Abstract. The packaging of bacteriophage PI DNA is initiated when the phage packaging site (pac) is recognized and cleaved and continues until the phage head is full. We have previously shown that pac is a 162-base-pair segment of P1 DNA that contains seven DNA adenine methyltransferase methylation sites (5′-GATC).
What are the characteristics of phage P1?
In addition to its generalized transducing abilities, phage P1 has two other features that are unusual among bacteriophage: First, though a temperate phage, P1 does not integrate its dsDNA genome into the bacterial genome. Instead, the genome circularizes and replicates in a plasmid-like state.
What is Khan P1 phage?
– Biotech Khan P1 is a temperate bacteriophage (phage) that infects Escherichia coli and a some other bacteria. When undergoing a lysogenic cycle the phage genome exists as a autonomous plasmid, that is maintained at low copy number, in the bacterium.
Can P1 phages infect all E coli strains?
As P1 phages can infect all E. coli strains, it is advisable to use a separate bench and equipment for all work related to phages. The virulent P1 phage mutant, P1vir ( Ikeda & Tomizawa, 1965 ), should be acquired from a supplier (e.g., CGSC, The Coli Genetic Stock Center).
Can phage P1 transduce chloramphenicol-and mercury-resistance genes to E coli?
Zeph et al. (1988) showed the transduction of chloramphenicol- and mercury-resistance genes to E. coli by phage P1 in sterile and nonsterile soils. The transductants were confirmed by heat induction of phage P1 Cm cts::Tn501 and with a biotinylated DNA probe ( Zeph and Stotzky, 1989; Zeph et al., 1991 ).