What is pREP4 plasmid?
Plasmid: pREP4 coli NM522, E. Plasmid contains the Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) to permit extrachromosomal replication in human, primate and canine cells. Hygromycin B resistance gene can be used for stable selection in transfected cells.
What is Addgene plasmid?
Addgene is a global, nonprofit repository that was created to help scientists share plasmids. Plasmids are DNA-based research reagents commonly used in the life sciences. When scientists publish research papers, they deposit their associated plasmids at Addgene.
What is autonomous plasmid?
Unlike conjugative plasmids that are extra-chromosomal and replicate autonomously, ICEs are integrated in the chromosome and replicate passively during chromosomal replication. It is generally thought that ICEs do not replicate autonomously.
Is Addgene a company?
Addgene is a unique, nonprofit whose mission is to accelerate research and discovery by improving access to useful research materials and information.
How do you order plasmids from Addgene?
How do I place an order? Does Addgene accept orders by fax, phone or email?
- Login to your Addgene account.
- Find the items you need in our online catalog and add them to your cart.
- Use our simple checkout process to complete your order online and enter payment information.
What is autonomous replication of plasmid?
Plasmids are autonomously replicating extrachromosomal elements that facilitate molecular studies in bacteria as well as in yeasts and other fungi (1).
What is meant by autonomous replication?
The process by which a DNA molecule is duplicated.
What is a vector and plasmid?
Plasmid and vector are two types of double-stranded DNA molecules that have different functions in the cell. The main difference between plasmid and vectors is that plasmid is an extra-chromosomal element of mainly bacterial cells whereas vector is a vehicle that carries foreign DNA molecules into another cell.
What is plasmid vector BYJU’s?
Plasmids are extrachromosomal DNA molecules. They are small, circular and have the ability to replicate autonomously. Replication of plasmid is not under the control of chromosomal DNA.
How do you cite Addgene website?
The plasmids you have received were created by your colleagues. Please acknowledge the Principal Investigator, cite the article listed on the plasmid datasheet, and include the Addgene plasmid number in future publications.
Can I buy plasmids?
you can share your own plasmid also you can purchase your desire plasmid. i myself purchased plasmid from addegene 68$ us dollar.