How many base pairs is pUC19?

2686 base pairs
The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).

What are pBR322 and pUC19?

pBR322 and pUC19 are two types of plasmid vectors. pBR322 is a plasmid vector that is 4361 base pairs in length, while pUC19 is a plasmid vector that is 2686 base pairs in length. So, this is the key difference between pBR322 and pUC19.

How can you use the EcoRI restriction enzyme to tell you if the gene has been inserted?

a) How can you use the EcoRI restriction enzyme to tell you if the gene has been inserted? You can cut the plasmid with EcoRI and look for two fragments, one that represents the vector and one that represents the insert. You would not know for sure that the insert is the harE gene without further tests.

What is pUC19 DNA?

Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.

Is pUC19 ampicillin resistant?

(A) The bla gene in pUC19, which confers ampicillin resistance, was replaced with fabI and its promoter region (pFab). The pUC19 multiple cloning site (MCS) is retained, however HincII, HindIII and PstI are not unique in pFab.

What is EcoRI mention its function?

EcoRI (pronounced “eco R one”) is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system.

Why does pUC19 have the ori segment?

The ori site, or origin of replication, is derived from the plasmid pMB1. pUC19 is small but has a high copy number. The high copy number is a result of the lack of the rop gene and a single point mutation in the ori of pMB1. The lacZ gene codes for β-galactosidase.

What is the host of plasmid pUC19?

Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.

What is pUC19?

View sequence details pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.

How many base pairs are in pUC19?

The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).

Where can I find a plasmid clone for use in pUC19?

pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1). NEB offers a selection of common cloning plasmids and DNAs for use as substrates. DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Is the MCS reversed in pUC18?

The MCS is reversed in pUC18. Sticky ends from different EcoO109I sites may not be compatible. Sticky ends from different TsoI sites may not be compatible. After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.