What Agar does Legionella grow on?

BCYE agar
Legionella is best grown on BCYE agar containing 0.1% α-ketoglutarate and L-cysteine supplemented with dyes to aid identification of colonies and antimicrobial agents such as vancomycin, polymyxin B, and anisomycin to inhibit overgrowth by other bacteria and yeast; incubation is at 35°C in a humidified atmosphere …

Will Legionella grow on blood agar?

Organisms that grow on blood agar as well as Legionella media are not legionellae. Some thermophilic spore-bearing organisms mimic Legionella colonies after 35°C incubation.

Does Legionella grow on chocolate agar?

Numerous reports in the literature have described Legionella isolation on selective supplemented media such glycine-vancomycin-polymyxin B-cycloheximide agar or BCYE supplemented with glycine, vancomycin, polymyxin B, and anisomycin, but only a few have described Legionella isolation on routinely used enriched media …

What type of samples for Legionella should not be concentrated?

Potable waters generally have low bacterial densities and are either cultured directly or concentrated to detect legionellae. Non-potable waters, such as those from cooling towers, generally do not require concentration because of their high bacterial concentrations.

Does Legionella require isolation?

The urinary antigen test detects Legionella pneumophila serogroup 1, the most common cause of Legionnaires’ disease; isolation of Legionella by culture is important for detection of other species and serogroups and for public health investigation.

Does Legionella show up on Gram stain?

Legionella are obligatory aerobic, fastidious, gram-negative bacilli that stain poorly with Gram stain (see Chapter 17, Figure 17-4) and grow poorly on conventional media. L. micdadei and some other Legionella species may stain weakly acid-fast.

Which plating media for Legionella micdadei and Legionella bozemanii?

Legionella micdadei and several strains of Legionella bozemanii show a preference for BCYE with 1.0% albumin (ABCYE) over standard BCYE. The inclusion of ABCYE in the regimen of plating media may be beneficial when clinical evidence indicates that a species other than Legionella pneumophila may be the causative agent.

What is the base media for the recovery of Legionella?

Buffered charcoal yeast extract (BCYE) agar containing 0.1% alpha-ketoglutarate is the base medium used for the recovery of Legionella from environmental and clinical specimens (Appendix 1).

Can biological quantification of potable and non-potable water samples detect Legionella?

This investigation compared biological quantification of potable and non-potable (cooling) water samples using pour plate heterotrophic plate count (HPC) methods and adenosine triphosphate (ATP) concentration measurement using bioluminescence. The relationship between these measurements and the presence of Legionella spp. was also examined.

How long should a negative plate be reincubated for Legionella infection?

Since Legionella is a relatively slow-growing bacterium, negative plates should be reincubated until 7 days post-inoculation and then re-examined for Legionella colonies. Discard plates after the seventh day.