What is the principle of ion chromatography?
Ion Chromatography is a method for separating ions based upon their interactions with resin (stationary phase) and the eluent (mobile phase). These phases differ between an anion column, which attracts anions, and a cation column, which attracts cations.
What are the 4 principles of chromatography?
Four separation techniques based on molecular characteristics and interaction type use mechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography.
What is the difference between ion exchange chromatography and affinity chromatography?
The key difference between affinity and ion exchange chromatography is that we can use affinity chromatography to separate charged or uncharged components in a mixture whereas we can use ion exchange chromatography to separate charged components in a mixture.
What is gradient elution in ion exchange chromatography?
Gradient elution is a powerful technique in ion chromatography. By varying the concentration of the eluant, ions with widely differing affinities for the separator resin can be eluted in one run. However, just as in other forms of chromatography, gradient elution places certain restrictions on the choice of eluant.
What are the types of ion exchange resins?
2.1. Ion exchange resins consists of two main types, i.e., cation exchange resins, that exchange positively charged ions, such as sodium, for calcium, and anion exchange resins, that exchange negatively charged ions, such as chloride, for arsenic.
What is the principle of ion exchange chromatography?
Ion Exchange chromatography Principle. The charged molecules in the sample are separated by the electrostatic forces of attraction when passed through an ionic resin at particular pH and temperature.
What are the advantages and disadvantages of ion exchange chromatography?
Advantages of ion exchange chromatography It is one of the most efficient methods for the separation of charged particles. It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids.
What is the principle of separation in chemistry?
The principle of separation is thus by reversible exchange of ions between the target ions present in the sample solution to the ions present on ion exchangers. In this process two types of exchangers i.e., cationic and anionic exchangers can be used.
How do you separate cations and anions in a column chromatography?
To separate anions “Anionic exchanger” is used, to separate cations “Cationic exchanger” is used. First the column is filled with ion exchanger then the sample is applied followed by the buffer. The tris-buffer, pyridine buffer, acetate buffer, citrate and phosphate buffers are widely used.